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KMID : 1007520110200061735
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Food Science and Biotechnology
2011 Volume.20 No. 6 p.1735 ~ p.1741
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Black Soybean (Glycine max cv. Heugmi) Seed Coat Extract Suppresses TPA or UVB-induced COX-2 Expression by Blocking Mitogen Activated Protein Kinases Pathway in Mouse Skin Epithelial Cells
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Jeon Ae-Ji
Lim Tae-Gyu
Jung Sung-Keun
Lee Eun-Jung
Yeom Myeong-Hun
Park Jun-Seong
Choung Myoung-Gun
Lee Hyong-Joo
Lim Yoong-Ho
Lee Ki-Won
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Abstract
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Although previous studies have reported that black soybean has chemopreventive potential, the underlying mechanisms remain unclear. Upregulation of cyclooxygenase- 2 (COX-2) has been known to be a key mediator in the development of skin cancer. The present study investigated the effect of black soybean (Glycine max cv. Heugmi) seed coat extract (BSE) on 12-O-tetradecanoylphorbol-13- acetate (TPA) or ultraviolet-B-(UVB)-induced COX-2 expression, and its underlying mechanisms. The TPA- or UVB-induced COX-2 expression in mouse skin epithelial cells were dose-dependently inhibited by BSE treatment. BSE suppressed the COX-2 promoter activity. BSE also attenuated the transactivation of activator protein-1 (AP-1) and nuclear factor (NF)-κB, transcription factors of COX- 2 expression in mouse skin epithelial cells transfected stably with AP-1 and NF-κB luciferase promoter, respectively. Furthermore, BSE inhibited the activation of MAPKKs/ MAPKs pathways that otherwise induced by TPA or UVB. Collectively, BSE suppresses TPA or UVB-induced COX- 2 expression by blocking the expressions of MAPKKs/ MAPKs pathways, which may contribute to its chemopreventive potential.
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KEYWORD
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black soybean seed coat extract, chemoprevention, cyclooxygenase-2, mitogen-activated protein kinase
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